Mapping gold‐labeled receptors on cell surfaces by backscattered electron imaging and digital image analysis: Studies of the IgE receptor on mast cells
作者:
R. F. Stump,
J. R. Pfeiffer,
M. C. Schneebeck,
J. C. Seagrave,
J. M. Oliver,
期刊:
American Journal of Anatomy
(WILEY Available online 1989)
卷期:
Volume 185,
issue 2‐3
页码: 128-141
ISSN:0002-9106
年代: 1989
DOI:10.1002/aja.1001850206
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractThe response of cells to signalling molecules such as hormones, growth factors, and immune mediators that bind to cell‐surface receptors depends in part on the density and distribution of the relevant receptors. We have developed methods to map the distribution of IgE receptors on RBL‐2H3 mast cells at high resolution in the scanning electron microscope (SEM). The key elements of our procedure are a new fixative that preserves receptor binding activity; a family of colloidal gold‐conjugated probes that bind directly or indirectly to the IgE‐receptor complex; an SEM with detectors for both secondary and backscattered electrons (to observe surface topography and gold particles, respectively); and an image processor that can average, digitize, and store these images. Topographical maps are generated by processing and superimposing the digitized images. The methods we describe can be applied to study the density and distribution of any membrane receptor that can be labeled with colloidal gold pa
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