IL3-Dependent Cells Die by Apoptosis on Removal of their Growth Factor
作者:
CromptonTessa,
期刊:
Growth Factors
(Taylor Available online 1991)
卷期:
Volume 4,
issue 2
页码: 109-116
ISSN:0897-7194
年代: 1991
DOI:10.3109/08977199109000262
出版商: Taylor&Francis
关键词: Apoptosis;IL3;Haemopoiesis
数据来源: Taylor
摘要:
AbstractThe IL3-dependent cell line FDCP-2 dies within 32 h of removal of IL3. Electron microscope studies indicate that 22 h after IL3 removal the nuclei are condensed, but the morphology of mitochondria and ribosomes is preserved. This pattern is characteristic of apoptosis. IL3 removal also results in the fragmentation of DNA into nucleosome-sized pieces, suggesting that an endonuclease is activated. The protein synthesis inhibitor, cycloheximide, enhances survival on IL3 removal, suggesting that death is an active process. The nuclease inhibitor, aurintricarboxylic acid, also enhances survival, suggesting a causal role for DNA fragmentation in apoptosis.
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