Thermally‐induced cell lysis inEscherichia coliK12
作者:
Jorge Membrillo‐Hernández,
Alejandra Núñez‐De La Mora,
Tania Del Rio‐Albrechtsen,
Rafael Camacho‐Carranza,
M. Carmen Gomez‐Eichelmann,
期刊:
Journal of Basic Microbiology
(WILEY Available online 1995)
卷期:
Volume 35,
issue 1
页码: 41-46
ISSN:0233-111X
年代: 1995
DOI:10.1002/jobm.3620350112
出版商: Wiley‐VCH
数据来源: WILEY
摘要:
AbstractEscherichia colicells exposed to high temperatures exhibit a progressive loss of viability. We observed two mechanisms of cell death induced by lethal temperatures: with and without lysis. The number of cells lysed by heat decreased at later stages of the growth curve, when cells were pre‐treated at lower temperatures for 10 minutes and when cells were pre‐treated with novobiocin, nalidixic acid and cadmium chloride. Cell lysis was similar in wild type,rpoH,groE anddnaK mutant cells as well as in cells which overproduce heat shock proteins GroE or DnaK. Results using cells aligned for cell division and cells growing at 42°C, 45°C and 47°C suggest that cells near division are more sensitive to lysis and that a high concentration of heat‐shock proteins increases their resistance
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