The elimination of viruses from Narcissus tazetta cv. Grand Soleil d'Or, and rapid multiplication of virus‐free clones
作者:
OLWEN M. STONE,
期刊:
Annals of Applied Biology
(WILEY Available online 1973)
卷期:
Volume 73,
issue 1
页码: 45-52
ISSN:0003-4746
年代: 1973
DOI:10.1111/j.1744-7348.1973.tb01308.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
SUMMARYArabis mosaic virus and narcissus degeneration virus were eliminated fromNarcissus tazettaL. cv. Grand Soleil d'Or by meristem‐tip culture. Cultures grew equally well in two liquid nutrient media, but tubes 25times75 mm size and temperaturec.12oC were optimal for growth and survival. Virus‐free bulbs grew more vigorously than infected bulbs, and gave more and larger flowers with a better colour. To increase the normal multiplication rate, either sector cuts were made in the basal plate, or bulbs were divided into chips(c.1 g), before planting. Multiplication was greater by chip‐cutting. Five virus‐free bulbs were propagated to over 2000 in 8 years; theoretically, 20000000 bulbs should be produced in a further 5–6 years, sufficient to replant the Isles of Scilly with virus
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