Evaluation of deep subsurface sampling procedures using serendipitous microbial contaminants as tracer organisms
作者:
RalphE. Beeman,
JosephM. Suflita,
期刊:
Geomicrobiology Journal
(Taylor Available online 1989)
卷期:
Volume 7,
issue 4
页码: 223-233
ISSN:0149-0451
年代: 1989
DOI:10.1080/01490458909377868
出版商: Taylor & Francis Group
关键词: Tracer microorganisms;coliforms;drilling muds;subsurface sampling
数据来源: Taylor
摘要:
Surface microbiological investigations are critically dependent on the procedures used to collect samples for study. It can be difficult to distinguish between indigenous organisms and those encountered as contaminants during the drilling process. We found that coliform bacteria contaminated drilling mud slurries. These bacteria proved useful as tracer organisms in evaluating the degree of microbial contamination accidentally encountered while drilling for subterranean samples. While these organisms were found in high numbers in both the circulating muds and in the mud reservoir, few subsurface samples harbored conforms. Subsurface slurries did not inhibit the growth of a known coliform inoculum. These results indicate that the methods used to collect and field‐process cores from Atlantic coastal plain sediments were sufficient to prevent a large degree of bacterial contamination in most samples. The microflora in drilling fluids did not quantitatively or qualitatively account for the number and diversity of bacteria in subsurface samples. We conclude that a large and viable bacterial community is present in deep regions of the terrestrial subsurface.
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