Light intensity from 2200 to 43 000 lux during production of urediospores ofPuccinia helianthihad little influence on germinability of fresh spores. The higher light intensities had an adverse effect on germinability of spores stored at −16 °C for 2 months. Daylength had little consistent effect on germinability of fresh or stored spores. Spores produced at day temperatures of 16, 21, and 27 °C showed no effect of temperature on germinability when fresh. After 2 months storage at −16 °C germination was highest for spores produced at 21°. Spores of races 1 and 3 germinated well after 1218 days of storage at −16°, and a small percentage germinated after storage at 4 °C. Races 2 and 4 germinated well after 1136 days storage at −16 and 4 °C. Races 2 and 4 survived longer than races 1 and 3 at 30° and at 23 °C, but not at 13 °C. Race 3 appeared more susceptible than race 1 to effects of light, temperature, and storage.