A method for light microscopy examination of cellular and structural interrelations in undemineralized tooth specimens
作者:
BjørndalLars,
ThylstrupAnders,
EkstrandKim Rud,
期刊:
Acta Odontologica Scandinavica
(Taylor Available online 1994)
卷期:
Volume 52,
issue 3
页码: 182-190
ISSN:0001-6357
年代: 1994
DOI:10.3109/00016359409027594
出版商: Taylor&Francis
关键词: Dentin;enamel;mineralization;odontoblasts;pulp cells
数据来源: Taylor
摘要:
This study examines ameloblasts and odontoblasts in relation to mineralizing enamel and dentin in thin undemineralized tooth sections from unerupted and partly erupted human third molars. The material comprised 12 freshly removed third molars. After fixation for 24 h the specimens were dehydrated and embedded in hydroxyethylmethacrylate. Undemineralized sections 10-15 pm thick were prepared with a cutting-grinding system, and radiomicrogxaphs were taken. Postsecretory ameloblasts were seen in different morphologic stages ranging from ruffle- and smooth-ended ameloblasts to cuboidal and squamous cells adhering to incomplete and fully mineralized enamel. The former occurred in the most coronal tooth part, whereas the latter were seen cervically. It was not possible to identify systematic modulation cycles as seen in postsecretory rat incisor ameloblasts. 'The well-defined subodontoblastic region with cell-free and cell-rich layers was established before tooth eruption. The function of the subodontoblastic region is not fully understood, but our findings suggest that the establishment of the region plays a role in maintaining the specific odontoblast phenotype in the coxonal region possibly linked to progressive dentin mineralization.
点击下载:
PDF (911KB)
返 回