The Breckenridge Sanitation District discharges tertiary-treated sewage effluent into Lake Dillon, Colorado, a major water supply reservoir for Denver. The effluent plume was mapped using rhodamine-WT dye during ice-covered and open-water conditions and compared to a criterion issued in a U.S. Environmental Protection Agency policy statement regarding mixing-zone dilution. Rhodamine-WT dye was used as a tracer and was injected directly into the sewage effluent at the outlet of the treatment plant. Dye concentrations were determined using fluorometric analysis. Dye-concentration isolines were determined by interpolation of a rectangular sampling grid. At all sampling locations, plateau dye concentrations were reached within 48 hours after injection began. Results indicated that the Breckenridge Sanitation District's effluent mixing zone extended 158.5 m (520 ft) into the lake during ice-covered conditions on March 15, 1995 (the date of maximum effluent discharge during the study period) and extended 64 m (210 ft) during open-water conditions on September 13, 1995. The EPA criterion for lakes specifies that the maximum allowable length of a mixing zone not exceed 61-m (200 ft).