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Classification of plant somatic embryos by computer vision

 

作者: Jari J. Hämäläinen,   Ulrika Kurtén,   Veli Kauppinen,  

 

期刊: Biotechnology and Bioengineering  (WILEY Available online 1993)
卷期: Volume 41, issue 1  

页码: 35-42

 

ISSN:0006-3592

 

年代: 1993

 

DOI:10.1002/bit.260410106

 

出版商: Wiley Subscription Services, Inc., A Wiley Company

 

关键词: pattern recognition;machine vision;tissue cultures;Betula pendulaRoth

 

数据来源: WILEY

 

摘要:

AbstractThis article deals with the automation of the process of somatic embryogenesis for the propagation of plants. An important problem is the monitoring of the embryo production process in order to decide the time to start harvesting embryos for further processing. The classification algorithm development for somatic embryos of birch (Betula pendulaRoth) showed that automated recognition of embryos at different developmental stages is possible. No globular stage embryos were classified to be heart or torpedo stage and no heart or torpedo stage embryos were classified to be at globular stage. Heart and torpedo stage embryos were classified into three developmental classes by a new index that describes the relation of embryo breadth to the length of the root. The probability of classifying a nonembryo as an embryo was less than 1%, and 14% of the object classified as embryos by a human expert were discarded by the algorithm. A computer vision system suitable for automated monitoring of samples from the bioreactor was constructed. © 1993 John Wiley&Sons, Inc

 

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