Purpose.To determine the efficacy of autologous serum drops in the treatment of superior limbic keratoconjunctivitis (SLK).Methods.Twenty-two eyes of 11 patients were diagnosed with SLK. All eyes were treated with 20% diluted autologous serum eyedrops 10 times a day in addition to ongoing treatment of dry eye. Fluorescein and rose bengal staining scores, as well as subjective symptom gradings, were performed before and after 4 weeks of therapy.Results.Nine of the 11 patients responded well to treatment (82%). The average rose bengal and fluorescein score improved (p< 0.05), and there was subjective improvement.Conclusion.Autologous serum application can be used as an alternative mode of therapy in SLK.