A Potent Stimulator of Small Intestinal Cell Proliferation Extracted by Simple Diffusion from Intact Irradiated Intestine:In VitroStudies
作者:
PottenC. S.,
BoothC.,
ChadwickC. A.,
EvansG. S,
期刊:
Growth Factors
(Taylor Available online 1994)
卷期:
Volume 10,
issue 1
页码: 53-61
ISSN:0897-7194
年代: 1994
DOI:10.3109/08977199409019603
出版商: Taylor&Francis
关键词: intestine;growth factor;proliferation;IEC 18 cells
数据来源: Taylor
摘要:
AbstractThe epithelium lining the small intestine is one of the most rapidly proliferating body tissues yet it rarely develops cancers. The proliferation, migration and differentiation of the stem cell progeny appears to be under very strict control. After 8 Gyγirradiation the murine epithelium contains surviving stem cells from which the epithelium rapidly and effectively regenerates, presumably in response to stimulatory signals, and then returns to steady state conditions after overshoots in proliferation. Here we describe the isolation and preliminary characterisationin vitroof a potent stimulatory extract obtained by diffusion from intact murine small intestine, post-irradiation.In addition toin vivoresponses the extract stimulates intestinal epithelial lines very effectively, most notably the rat IEC 18 line where it can replace the serum requirement. The extent of the induced increase in proliferation could not be reproduced by any other single growth factor tested. Preliminary evidence suggests the extract contains either a potent stimulatory cocktail of factors or a novel intestinal growth factor(s).
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