Procedures for the extraction, separation and estimation of the major fat‐soluble pigments of hay
作者:
J. Davidson,
期刊:
Journal of the Science of Food and Agriculture
(WILEY Available online 1954)
卷期:
Volume 5,
issue 1
页码: 1-7
ISSN:0022-5142
年代: 1954
DOI:10.1002/jsfa.2740050101
出版商: John Wiley&Sons, Ltd
数据来源: WILEY
摘要:
AbstractNew methods are described for the extraction, chromatographic separation and spectro‐photometric identification and estimation of chlorophyll‐a, chlorophyll‐b, phaeophytin‐a, phaeophytin‐b, carotene and xanthophyll in hay. The most suitable solvent for extraction was 85% acetone. The chlorophylls were separated chromatographically from the phaeophytins, carotene and xanthophyll on a sucrose‐sodium sulphate mixture. The chlorophyll‐aand chlorophyll‐bwere estimated simultaneously in the green solution, and phaeophytin‐aand phaeophytin‐bwere estimated simultaneously in the yellow solution, by spectrophotometry. Carotene and, subsequently, xanthophyll were separated chromato‐graphically from the yellow solution on magnesium oxide, and were
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