Molecular Cloning of Two Novel Transmembrane Ligands for Eph-Related Kinases (LERKS) that are Related to LERK-2
作者:
NicolaNicos A.,
VineyElizabeth,
HiltonDouglas J.,
RobertsBronwyn,
WillsonTracy,
期刊:
Growth Factors
(Taylor Available online 1996)
卷期:
Volume 13,
issue 1-2
页码: 141-149
ISSN:0897-7194
年代: 1996
DOI:10.3109/08977199609034574
出版商: Taylor&Francis
关键词: LERK;ERK;tyrosine kinase receptors;orphan receptors
数据来源: Taylor
摘要:
AbstractA search of the nucleic acid database of expressed sequence tags (ESTs) revealed several partial cDNA sequences that could encode proteins homologous to the ligands for Eph-related kinases (LERKs). Oligonucleotides designed from the ESTs were used to probe a human brain cDNA library and obtain overlapping clones that encoded two different novel LERKS (NLERK-1 and NLERK-2). NLERK-1 and NLERK-2 are most closely related to human LERK-2/Elk-ligand and they form a subclass of LERKs that contain a transmembrane domain and a conserved cytoplasmic domain. Full-length NLERK-1 was expressed as a glycosylated membrane protein in COS cells and was not secreted into the medium. Full-length NLERK-2 was similarly expressed in COS cells but both membranebound and a truncated, proteolytically-released form were detected. Engineered forms of both NLERK-1 and NLERK-2 lacking transmembrane and cytoplasmic domains were also expressed in COS cells and each was detected in the extracellular medium.
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