Differential distribution of glutamylated tubulin during spermatogenesis in mammalian testis
作者:
Jean‐Pierre Fouquet,
Marie‐Louise Kann,
Bernard Edde,
Annie Wolff,
Elisabeth Desbruyeres,
Philippe Denoulet,
期刊:
Cell Motility and the Cytoskeleton
(WILEY Available online 1994)
卷期:
Volume 27,
issue 1
页码: 49-58
ISSN:0886-1544
年代: 1994
DOI:10.1002/cm.970270106
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: spermatozoa;centriole;axoneme;immunogold;acetylated tubulin;tubulin heterogeneity
数据来源: WILEY
摘要:
AbstractThe distribution of glutamylated tubulin has been analyzed in mammalian testis using the specific mAb GT335 by immunoelectron microscopy and immunoblotting. In spermatozoa of various species, immunogold labeling showed the presence of glutamylated tubulin in all of the microtubules of axoneme and centrioles, whereas the microtubule network of the spermatid manchette was unlabeled. In earlier germ cells, centriole was the only microtubule structure to be labeled. A similar distribution was observed using the anti‐acetylated tubulin antibody (6‐11B‐1), confirming previous results of Hermo et al. [Anat. Rec. 229:31‐50, 1991]. However, among testicular somatic cells, microtubules of some Sertoli cell branches were not acetylated but glutamylated. 2‐D PAGE of mouse and hamster sperm extracts showed a high level of α and β‐tubulin heterogeneity, comparable to that found in brain. Immunoblotting with GT335 revealed a large amount of glutamylated tubulin resolved into numerous α as well as β‐tubulin isoforms. This suggests that the major testis‐specific tubulin isotypes (mα3/7 and mβ3) are also glutamylatable. These results show a subcellular sorting of posttranslationally modified tubulin isoforms in spermatids, glutamylation being associated with the most stable microtubule structures.
点击下载:
PDF
(1068KB)
返 回