Purpose.To determine the effect of scleral fixation ring placement in induction of astigmatism by topographic analysis.Methods.Baseline measurements were used in human eyes from the Utah Lions Eye Bank. In two control eyes, the effect of placing and replacing the eye in the holder was determined. In nine eyes, the effect of placing a 14-mm ring 2.0-mm from the limbus and an 18-mm ring 4.0-mm from the limbus, both in line with and between the rectus muscles, was determined. Induced astigmatism (IA) was determined by Alpins' vector analysis method.Results.Control eyes had a mean variation of 0.26 ± 0.18 diopters (D) (range, 0.07–0.65). The study eyes had a mean IA of 1.55 D with statistically significant differences noted when comparing 14-mm rings (0.92 ± 2.00 D) versus 18-mm rings in line with the rectus (3.02 ± 1.49 D;p= 0.02), with 18-mm rings in line with the rectus muscles (3.02 ± 1.46 D) versus between the muscles (1.37 ± 1.14 D;p= 0.03), and all measurements with the 14-mm ring (1.0 ± 1.49 D) versus the 18-mm ring (2.14 ± 1.51 D;p= 0.04.).Conclusions.Significant IA can occur with placement of fixation rings, especially when suture placement is near the insertions of the rectus muscles.