A Rapid and Simple Microassay for Lidocaine in Human Blood Plasma Using Gas‐Liquid Chromatography with Nitrogen Detection
作者:
J. Gal,
M. Freedman,
E. Kumar,
C. Freed,
期刊:
Therapeutic Drug Monitoring
(OVID Available online 1981)
卷期:
Volume 3,
issue 2
页码: 177-180
ISSN:0163-4356
年代: 1981
出版商: OVID
关键词: Lidocaine;Assay;Nitrogen-detection gas-liquid chromatography
数据来源: OVID
摘要:
Lidocaine is widely used in antiarrhythmic therapy, and the need to monitor the drug concentration in the blood of patients receiving lidocaine is well established. We have developed a new assay for lidocaine designed for the routine clinical therapeutic drug monitoring laboratory. A 100-μl aliquot of blood plasma is treated with 50 μl of internal standard solution and the mixture alkalinized. The drug and internal standard are extracted with toluene and analyzed by nitrogen-detection gas-liquid chromatography. A single extraction is used, and no solvent evaporation is required, resulting in a simple and rapid procedure. Using the single-point standard, the within-day coefficient of variation (CV) was 6.4%, while the between-day CV was 7.4%. Monoethylgly-cinexylidide, the major plasma metabolite of lidocaine, and several drugs potentially present in patients receiving lidocaine were shown not to interfere. The procedure has significant advantages over previously published similar methods.
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