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Identification of Basic Nuclear Proteins by their Boronate Complex

 

作者: JobstK.,   LakatosÁ.,   HorváthA.,  

 

期刊: Biotechnic&Histochemistry  （Taylor Available online 1992）
卷期: Volume 67, issue 3  

页码: 158-160

 

ISSN:1052-0295

 

年代: 1992

 

DOI:10.3109/10520299209110027

 

出版商: Taylor&Francis

 

关键词: histone-blocking;histone-CDI;histone-boronate;basic proteins;toluidine blue

 

数据来源: Taylor

 

摘要:

A new histochemical reaction for the identification of histone type basic proteins has been developed. Carbonyldiimidazol is used to activate the basic proteins of TCA-extracted nuclei, their m-aminophenylboronic acid complex is prepared, and the DNA-free, histone-containing nuclei are stained with toluidine blue at pH 5.5.

 

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