α1‐Adrenoceptor Stimulation Enhances the Delayed Rectifier K+Current of Guinea Pig Ventricular Cells Through the Activation of Protein Kinase C
作者:
Noritsugu Tohse,
Haruaki Nakaya,
Morio Kanno,
期刊:
Circulation Research
(OVID Available online 1992)
卷期:
Volume 71,
issue 6
页码: 1441-1446
ISSN:0009-7330
年代: 1992
出版商: OVID
关键词: α1-adrenoceptors;delayed rectifier K+current;protein kinase C;cardiomyocytes;phenylephrine;H-7;patch clamp
数据来源: OVID
摘要:
The effect of α1-adrenoceptor stimulation on the delayed rectifier K+current (Ik) was examined in isolated guinea pig ventricular cells by use of the patch-clamp method. IKwas evoked by a 3-second depolarizing pulse from a holding potential of −30 mV in a Na+- and K+-free solution containing 3 μM nifedipine. Phenylephrine (30 μM) in the presence of propranolol (1 μM) produced an increase in IK. In five cells, phenylephrine increased the tail current of IKby 23±5%. This effect of phenylephrine was blocked by prazosin (0.3 μM), a selective α1-blocker. Phenylephrine produced only a small effect on the voltage and time dependence of IK. Pretreatment with 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7, 10 μM) abolished the phenylephrine-induced increase in IK. In addition, pretreatment with a maximally effective concentration of 12-O-tetradecanoylphorbol 13-acetate (100 nM) abolished the phenylephrine-induced increase in IK. In conclusion, α1-adrenoceptor stimulation increases IKin guinea pig cardiomyocytes. This cvl-adrenoceptor-mediated response may be related to an activation of protein kinase C. The increase in IKmay explain a shortening of action potential duration observed after α1-adrenoceptor stimulation in guinea pig cells.
点击下载:
PDF
(1017KB)
返 回