The extraction of uranium as well as Eu-(152 + 154), Co-60, Ru-103 and Cs-137 by di(2-ethylhexyl) phosphoric acid (HDEHP), tricaprylmethyl ammonium chloride (Aliquate-336) and tricapryltertiaryamine (Alamine-336) in kerosene from aqueous nitrate medium of constant ionic strength, 0.6 (H+, NaNO3) was investigated. The aqueous phase used is a representative medium for certain medium level waste (MLW) solutions. The obtained results indicate that HDEHP system is more suitable for the separation of U(VI) and Eu(III) from Ru(III), Co(II) and Cs(I). Using HDEHP loaded on XAD-4, chromatographic extraction was applied successfully for the recovery of uranium and Eu(152+154) from the aforementioned solution.