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Investigation of a Novel Microtiter Plate Support Material and Scanner Quantitation of Immunoassays, Proteins and Phospholipids

 

作者: StuartG. Reeves,   MatthewA. Roberts,   SuiTi A. Siebert,   RichardA. Durst,  

 

期刊: Analytical Letters  (Taylor Available online 1993)
卷期: Volume 26, issue 7  

页码: 1461-1476

 

ISSN:0003-2719

 

年代: 1993

 

DOI:10.1080/00032719308017425

 

出版商: Taylor & Francis Group

 

关键词: Solid-phase immunoassay;densitometry;Alachlor;immunomigration;liposomes;protein assay;ELISA

 

数据来源: Taylor

 

摘要:

A recently developed shallow-well microtiter plate, made from a specially formulated polymer that binds proteins, peptides and nucleic acids rapidly and efficiently, has been investigated for use in solid-phase immunoassays as well as for protein and phospholipid assays. The assay uses quantitation of the color intensity of a dye on the solid phase by means of a simple desk-top scanner coupled to a computer which allows the gray-scale density of the color to be easily and accurately measured. Consequently, this approach is independent of the absorption spectrum of the dye used.

 

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