Evaluation of protein‐denaturing immunoassays for avidity of immunoglobulin G to rubella virus
作者:
J. Polanec,
I. Seppälä,
S. Rousseau,
K. Hedman,
期刊:
Journal of Clinical Laboratory Analysis
(WILEY Available online 1994)
卷期:
Volume 8,
issue 1
页码: 16-21
ISSN:0887-8013
年代: 1994
DOI:10.1002/jcla.1860080105
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: antibody affinity;avidity ELISA;virus serology
数据来源: WILEY
摘要:
AbstractImmunoassays have been recently developed that measure the avidity of IgG antibodies to complex microbial antigens and are suitable for serologic diagnosis of infectious diseases. In these avidity ELISAs, proteindenaturing agents are applied either as diluents of patient sera to prevent the immune complexing of IgG (diluting principle), or the preformed complexes are treated with the protein denaturants (eluting principle). We compared four protein denaturants previously used in such assays, in a diagnostic avidity ELISA for rubella IgG. Diethylamine, guanidine, thiocyanate, or urea were applied, by either principle at various concentrations, and thiocyanate, or urea were applied, by either principle at various concentrations, and thiocyanate at an optimum pH. Patient sera obtained recently after primary infection were distinguished from sera representing past rubella immunity by any protein denaturant tested by the eluting principle, which was superior to the diluting principle. © 1994 Wiley‐Liss, I
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