Detection of pectinesterase in polyacrylamide gels
作者:
Jesús Alonso,
M. Teresa Rodríguez,
Wenceslao Canet,
期刊:
ELECTROPHORESIS
(WILEY Available online 1995)
卷期:
Volume 16,
issue 1
页码: 39-42
ISSN:0173-0835
年代: 1995
DOI:10.1002/elps.1150160109
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Pectinesterase;Isoelectric focusing;Native polyacrylamide gel electrophoresis
数据来源: WILEY
摘要:
AbstractA rapid and sensitive method of detecting pectinesterase activity following electrophoresis or isoelectric focusing in polyacrylamide gels is described. The method uses ruthenium red and requires no addition of substrate when making the gels, thus obviating direct enzyme‐substrate contact during electrophoresis. Because of its versatility, the method can be used in a wide variety of applications, such as plant and microbial taxonomy, enzyme purification and characterization, or as an analytical method in fresh and processed plant technolog
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