Efficient Propagation and Cloning of Human T Cells in the Absence of Antigen by Means of OKT3, Interleukin 2, and Antigen‐Presenting Cells
作者:
M. LONDEI,
B. GRUBECK‐LOEBENSTEIN,
P. BERARDINIS,
C. GREENALL,
M. FELDMANN,
期刊:
Scandinavian Journal of Immunology
(WILEY Available online 1988)
卷期:
Volume 27,
issue 1
页码: 35-46
ISSN:0300-9475
年代: 1988
DOI:10.1111/j.1365-3083.1988.tb02321.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
The analysis of T lymphocytes infiltrating tissues afflicted by autoimmune diseases may provide major clues towards understanding the pathogenesis of such diseases. Currently the best approach to studying heterogeneous populations such as T lymphocytes involves long‐term culture and cloning. In order to grow and clone T lymphocytes, regular restimulation with the specific antigen is essential, otherwise growth will stop and/or specificity may be lost. In autoimmune diseases the antigens involved in triggering the immunological reaction of T cells are usually unknown. Therefore an alternative way of stimulating T lymphocytes without loss of specificity is clearly needed. Here we describe the cloning and expansion of antigen‐specific T cell clones from the blood of a healthy donor to sizeable numbers of cells (>108) by means of anti‐CD3 monoclonal antibody and recombinant IL‐2 The results obtained showed that this approach can be used to clone and ‘expand’ T lymphocytes that retain antigen specificity over a prolonged period, in this case over 10 weeks. This technique has been used to clone and expand T lymphocytes infiltrating the affected tissues in a variety of autoimmune disorders such as Hashimoto's thyroiditis, Graves' disease, and rheumatoid arthritis, and is an efficient method of propagating T cells, by mimicking the antigen
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