首页   按字顺浏览 期刊浏览 卷期浏览 Epstein-Barr Virus-Encoded Nuclear Protein EBNA-3 Interacts With the ε-Subunit of ...
Epstein-Barr Virus-Encoded Nuclear Protein EBNA-3 Interacts With the ε-Subunit of the T-Complex Protein 1 Chaperonin Complex

 

作者: Elena,   Kashuba Katja,   Pokrovskaja George,   Klein Laszlo,  

 

期刊: Journal of Human Virology  (OVID Available online 1999)
卷期: Volume 2, issue 1  

页码: 33-37

 

ISSN:1090-9508

 

年代: 1999

 

出版商: OVID

 

关键词: EBNA;EBV;two-hybrid;chaperonin;TCP-1.

 

数据来源: OVID

 

摘要:

Objective:To find cellular proteins that associate with EBNA-3 (also called EBNA-3A), one of the Epstein-Barr virus (EBV)-encoded growth transformation-associated nuclear proteins.Methods:Screening human cDNA libraries in the yeast two-hybrid system and performing an analysis of interaction in vitro as well as in cell lysates.Results:EBNA-3 binds to the epsilon subunit of the chaperonin containing T-complex protein 1 (ε-TCP-1) in the yeast two-hybrid system. The cDNA clone isolated from a human lymphocyte library was found to encode the middle and Cterminal part of ε-TCP-1. The interaction was confirmed by showing that a GST fusion protein specifically precipitated EBNA-3 from CV1 cells infected with recombinant vaccinia virus expressing EBNA-3. The interacting region was mapped to the putative apical domain of ε-TCP-1.Conclusions:This study shows that large, virus-encoded transforming proteins such as EBNA-3 may receive help for their initial folding by chaperonin complexes. The recognition of the chaperonin complex likely occurs through specific interaction with one of the subunits. We suggest that nascent EBNA-3 may recognize the TCP-1 complex by interacting with the apical region of the epsilon subunit.Journal of Human Virology 1999;2:33-37 © Lippincott Williams & Wilkins, Inc.

 

点击下载:  PDF (3304KB)



返 回