Effects of different epoxies on avoiding wrinkles in thin sections of botanical specimens
作者:
Kaarina Pihakaski,
Ulla‐Maija Suoranta,
期刊:
Journal of Electron Microscopy Technique
(WILEY Available online 1985)
卷期:
Volume 2,
issue 1
页码: 7-10
ISSN:0741-0581
年代: 1985
DOI:10.1002/jemt.1060020102
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Folds;Wrinkles;Epoxy resin;Thin section;Botanical specimen
数据来源: WILEY
摘要:
AbstractFold or wrinkles appear frequently in thin sections cut from botanical material embedded in epoxy resin such as Epon 812 (Polysciences) or EM‐bed‐812 (Electron Microscopy Sciences). Small wrinkles may occur more or less perpendicular to the cutting direction and mostly across the cell wall, causing problems in the examination of the ultrastructure. We studied the occurrence of wrinkles and folds by methodological sectioning, with regard to block hardness, use of Formvar‐support film, and chloroform vapor. Methodological sectioning or changing the block hardness did not reduce the occurrence of wrinkles in sections. Chloroform vapor improved the result as did also the use of uncoated grid. Wrinkles and folds could be avoided completely by embedding the specimens in LX‐112 (Ladd) or ERL‐4206 (Polysciences) low‐viscosity epoxy resins, in which case Formvar‐coated grids could be used, and chloroform vapor was
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