Silver Enhancement of Nickel-Diaminobenzidine as Applied to Single and Double Immunoperoxidase Staining
作者:
FrigoBarbara,
ScopsiLucio,
PatriarcaCarlo,
RilkeFranco,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 3
页码: 159-166
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110572
出版商: Taylor&Francis
关键词: immunocytochemistry;detection efficiency;diaminobenzidine;silver enhancement;argyrophilia
数据来源: Taylor
摘要:
A reliable and practical method is proposed for increasing sensitivity and detection efficiency of immunocytochemical techniques, based on silver enhancement of the nickel-diaminobenzidine product of the peroxidase reaction. The procedure produces a strong signal at the site of the end product of the peroxidase reaction which is visible as black grains at the light microscopic level. The method has been used to detect peroxidase labeled probes in immunocytochemical tissue preparations and blotting assays and is ideal for the purposes of double staining and photographic documentation.
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