A Consistent Method for Producing Gram Quantities of TypicalBipolaris ZeicolaandB. MaydisConidia
作者:
FoudinArnold S.,
CalvertOscar H.,
期刊:
Mycologia
(Taylor Available online 1987)
卷期:
Volume 79,
issue 1
页码: 117-122
ISSN:0027-5514
年代: 1987
DOI:10.1080/00275514.1987.12025376
出版商: Taylor&Francis
关键词: Bipolaris;sporulation;LBA medium
数据来源: Taylor
摘要:
ABSTRACTA consistent method was developed to economically produce gram quantities of typicalBipolaris zeicolaandB. maydisconidia that are viable, uniform, morphologically normal, and free from other fungi and bacteria. Such conidia were produced on a lima bean agar (LBA) consisting of 20 g/liter finely ground dry baby lima bean seed and 7.5 g/liter USP crude agar. Plates of LBA were inoculated with conidia, incubated at 25 C in the dark or under fluorescent light (continuous 100–200 lux) for 14 days, and dried at 32–35 C to a thin film. Conidia were aseptically vacuumed at 200 mm Hg from the dried medium with a cyclone spore collector. We obtained 25 g of phragmoconidia of each species from 300 LBA petri dish cultures (~82 mg conidia/plate culture). Cultures on the LBA medium produced four to six times more conidia than on potato dextrose agar medium and at the lowest practical cost of any of several substrates tried.
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