Production of H‐Y antibody in the ascites fluid of mouse and localization of the antigen on cells and tissues
作者:
Subramanian V. Iyer,
Tarala D. Nandedkar,
Umashashi C. Hegde,
期刊:
Gamete Research
(WILEY Available online 1989)
卷期:
Volume 22,
issue 1
页码: 37-49
ISSN:0148-7280
年代: 1989
DOI:10.1002/mrd.1120220105
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: H‐Y antigen;indirect immunofluorescence;immunohistochemistry
数据来源: WILEY
摘要:
AbstractA procedure is described for the production of large amounts of ascites fluid containing specific H‐Y antibody. The distribution of H‐Y antigen on mouse epididymal spermatozoa, thymocytes, and splenocytes was carried out using this specific antibody in the microcytotoxicity test and ELISA. Employing the indirect immunofluorescent technique, the H‐Y antigen was localized on the acrosomal membrane of mouse epididymal and washed ejaculated human spermatozoa and on the entire membrane of mouse splenocytes and thymocytes. Immunohistochemical localization of the antigen in the testicular section indicated its presence in the cytoplasm of Leydig cells and on the membrane of Sertoli cells and sperm
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