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Improved Ultra‐Thin Sectioning of Tissue for Electron Microscopy

 

作者: James Hillier,   Mark E. Gettner,  

 

期刊: Journal of Applied Physics  (AIP Available online 1950)
卷期: Volume 21, issue 9  

页码: 889-895

 

ISSN:0021-8979

 

年代: 1950

 

DOI:10.1063/1.1699778

 

出版商: AIP

 

数据来源: AIP

 

摘要:

The use of a reservoir of liquid to collect ribbons of thin sections as they are cut enables serial sections to be obtained and provides means for detecting variations in thickness. Reducing the effects of static friction and of external vibrations makes it possible to cut long series of sections 0.2&mgr; thick and uniform to better than five percent. Some observations which may throw some light on the mechanics of the cutting process are reported. Mounting the sections without any treatment is shown to be superior to any technique in which the embedding material is removed or replaced. It is shown that the technique provides sections in which a structural resolution of 200A is possible. The remaining artifacts introduced by the sectioning and mounting are easily recognized deformations which do not influence the interpretation.

 

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