Five air samplers (Millipore, all‐glass impinger, centrifugal, Andersen, and absorbent cotton) were evaluated for their ability to collect airborne grain particles contaminated with aflatoxin B1.Corn dust containing 100 μg aflatoxin B1/g was aerosolized within a containment system. Each device sampled 100 I air, thus exchanging the air in the chamber two times. Aflatoxin B1was extracted from all sampling matrices and was detected and quantitated with thin‐layer chromatography and scanning fluoro‐densitometry. The highest efficiency was obtained with the Millipore sampler, while the efficiencies of the centrifugal and the cotton samplers were almost identical. Efficiency of an Andersen was less, with no toxin recovered from an all‐glass impinger. Measurement of particle size was accomplished with the Andersen sampler