A Soap Technique for Cell Separation to Study the Seed Coat ofSesbania punicea
作者:
BevilacquaLetizia,
MassaGiorgio,
ModenesiPaolo,
FossatiFabrizia,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1993)
卷期:
Volume 68,
issue 3
页码: 159-160
ISSN:1052-0295
年代: 1993
DOI:10.3109/10520299309104688
出版商: Taylor&Francis
关键词: cell separation;soap;seed coat;Sesbaniapunicea
数据来源: Taylor
摘要:
A technique is described for separating plant cells used for morphological studies. The plant material is placed in a concentrated solution of olive oil castile soap for 1–2 days or more. The material is then thoroughly washed and placed between two glass slides. The upper glass slide is lifted from the lower one, then gently pressed down several times. Through this procedure Malpighian cells of the seed coat ofSesbania punicea. mesophyll cells ofEuphorbia peplusand ofTrifolium pratenseand cortical cells of the aerial roots ofMonstera deliciosahave been separated. Various shapes of the Malpighian cells of the Sesbaniapuniceaseed coat can be observed along with intermediates.
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