Membrane Mechanism Mediates Progesterone Stimulatory Effect on LHRH Release from Superfused Rat Hypothalami in vitro
作者:
Ferng-Chun Ke,
Victor D. Ramirez,
期刊:
Neuroendocrinology
(Karger Available online 1987)
卷期:
Volume 45,
issue 6
页码: 514-517
ISSN:0028-3835
年代: 1987
DOI:10.1159/000124784
出版商: S. Karger AG
关键词: Hypothalamus;Immobilized steroids;LHRH;Progesterone
数据来源: Karger
摘要:
To determine whether the plasma membrane is a primary site for progesterone (P4) action on the neural LHRH apparatus of hypothalamic tissues derived from ovariectomized, estradiol-primed (OVX + E2) immature rats, immobilized P4 was infused directly to these tissues using a superfusion technique. Two kinds of immobilized P4 with bovine serum albumin (BSA) conjugated at positions 3 or 11, or 11-deoxycorticosterone (DOC) immobilized at position 21 of the steroid molecule, respectively, were tested for structural specificity. Among the three immobilized steroids, only P4 with BSA conjugated at position 3 (P4–3-BSA) was effective in stimulating LHRH release in vitro. P4-3-BSA at 0.5 µg/ml, approximately 1.7 × 10–7M of P4, increased LHRH levels in the superfusates to about 2.5-fold those of pretreatment levels. In addition, no conversion of P4–3-BSA to free progesterone was detected. This observation demonstrated that the plasma membrane is a primary site for the stimulating effect of P4 on LHRH release from hypothalamic tissue i
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