Short‐Term Metabolism of Radioactive Kinetin during Lettuce Seed Germination
作者:
J. A. MIERNYK,
D. F. BLAYDES,
期刊:
Physiologia Plantarum
(WILEY Available online 1977)
卷期:
Volume 39,
issue 1
页码: 4-8
ISSN:0031-9317
年代: 1977
DOI:10.1111/j.1399-3054.1977.tb09276.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
AbstractExogenously applied 8‐14C‐kinetin is rapidly taken up by seeds of lettuce (Lactuca sativaL. cv. Grand Rapids). Radioactive metabolites were extracted and purified by solvent fractionation, column and paper chromatography. The primary metabolite was identified as the 9‐riboside‐5′‐monophosphate. As germination proceeds, some kinetin is released from this bound storage form, giving a maximum level of free kinetin at 12 hours after imbibition. After this time the concentration of ribotide increases while the concentration of free base decreases. Other metabolites are the 9‐riboside, AMP and IMP.It is suggested that a required amount of free base cytokinin is necessary by 12 hours after imbibition. This concentration of free cytokinin may act as a physiological trigger for later events durin
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