Origin of Intact Lactoferrin and Its DNA‐Binding Fragments Found in the Urine of Human Milk‐Fed Preterm Infants. Evaluation by Stable Isotopic Enrichment
作者:
T. HUTCHENS,
JOSEPH HENRY,
TAI-TUNG YIP,
DAVID HACHEY,
RICHARD SCHANLER,
KATHLEEN MOTIL,
CUTBERTO GARZA,
期刊:
Pediatric Research
(OVID Available online 1991)
卷期:
Volume 29,
issue 3
页码: 243-250
ISSN:0031-3998
年代: 1991
出版商: OVID
数据来源: OVID
摘要:
The origin of intact (78-kD) lactoferrin found in the urine of human milk-fed preterm infants was investigated using human milk containing proteins enriched with [13C]leucine and [15N2]lysine or [2H4]lysine. Mothers of infants selected for the study were infused i.v. with [13C]leucine and [15N2]lysine or [2H4]lysine to label milk proteins. The labeled milk was collected from each mother, pooled, fortified with a lyophilized human milk fraction, and fed to her preterm infant by continuous orogastric infusion for a period of 48 h. Urine was collected from each infant for 96 h. Intact lactoferrin (78 kD) and DNA-bioding lactoferrin fragments (51 and 39 kD) were purified from the urine by affinity chromatography on columns of immobilized single-stranded DNA-agarose. The concentration and isotopic enrichment of the intact lactoferrin and DNA-binding fragments were determined separately after their isolation by high-performance reverse-phase (phenyl) chromatgraphy. Mass spectral analyses indicated that the isotopic enrichment of the purified urinary lactoferrin was 87 to 100% of that in the labeled human milk lactoferrin. Similar results were obtained for the isolated DNA-binding lactoferrin fragments. The ratios of isotopically labeled leucine to lysine in the purified milk lactoferrins and urinary lactoferrins were similar for each mother/infant pair. Isotopically labeled lysine, added to the milk as free amino acid, was not incorporated into the purified urinary lactoferrin. These results demonstrate that undegraded (78-kD) lactoferrin of maternal origin is absorbed by the gut and excreted intact in the urine of preterm infants; nearly all of the urinary lactoferrin was of maternal origin. The possible immunoregulatory functions of the absorbed intact, maternal lactoferrin are discussed. (Pediatr Res 29: 243–250, 1991
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