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Acrylamide arrests mitosis and prevents chromosome migration in the absence of changes in spindle microtubules

 

作者: DaleW. Sickles,   DaveA. Welter,   MarvinA. Friedman,  

 

期刊: Journal of Toxicology and Environmental Health  (Taylor Available online 1995)
卷期: Volume 44, issue 1  

页码: 73-86

 

ISSN:0098-4108

 

年代: 1995

 

DOI:10.1080/15287399509531944

 

出版商: Taylor & Francis Group

 

数据来源: Taylor

 

摘要:

Cultured HT 1080 fibrosarcoma cells were exposed to acrylamide (ACR), an industrial neurotoxicant that disrupts neuronal intracellular transport, to determine if mitosis (another microtubule‐based intracellular transport system) was adversely affected. The number of cells arrested in mitosis increased, in a concentration‐dependent manner, from 1 to 10 mM acrylamide. A 4‐h exposure to 10 mM acrylamide increased the mitotic index by 4.5‐fold over control, comparable to the arrest caused by colchicine. In mitotic acrylamide‐exposed cells, the chromosomes remained at the metaphase plate; no changes in spindle micro‐tubules (MTs), as seen with tubulin immunofluorescence, were observed. The distance between spindle poles (interaster) was the same in control and experimental cells. The non‐neurotoxic analogue méthylène bisacrylamide had no effect in the same concentration range. The data suggest potential molecular mechanisms of action for general toxicity and neuro‐toxicity to be disruption in MT disassembly or MT‐kinetochore interactions and/or cellular homeostatis.

 

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