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Determination of Lactate Dehydrogenase by the Electrochemical Oxidation of Nadh at a Modified Microband Gold Electrode

 

作者: Chenxin Cai,   Huangxian Ju,   Hongyuan Chen,  

 

期刊: Analytical Letters  （Taylor Available online 1995）
卷期: Volume 28, issue 5  

页码: 809-820

 

ISSN:0003-2719

 

年代: 1995

 

DOI:10.1080/00032719508001426

 

出版商: Taylor & Francis Group

 

关键词: Amperometry;lactate dehydrogenase;nicotinamide adenine dinucleotide;microelectrode;chemically modified electrode

 

数据来源: Taylor

 

摘要:

A new amperometric electrode for the assay of lactate dehydrogenase (LDH) is prepared by modifying a nanometer—sized microband gold electrode with nickel hexacyanoferrate. The amperometric determination bases on electrochemically detecting NADH produced by the enzymatic reaction of lactate with NAD+under the catalysis of LDH. The modified electrode can sensitively detect LDH at +0.55V vs. SCE with a linear response in the range of 10U / mL to 350U / mL under optimum conditions.

 

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