Light and Scanning Electron Microscopy of Greenbug Aphid Damage in Wheat Using the Same Section
作者:
LedfordJoanna H.,
RichardsonPaul E.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1994)
卷期:
Volume 69,
issue 6
页码: 342-347
ISSN:1052-0295
年代: 1994
DOI:10.3109/10520299409106315
出版商: Taylor&Francis
关键词: greenbug;aphid;wheat;correlative microscopy;scanning electron microscopy;feeding damage
数据来源: Taylor
摘要:
A method has been developed to enable correlative light microscopy (LM) and scanning electron microscopy (SEM) on the same section of wheat(Triticum aestivumL.) leaves infested by greenbug aphids(Schizaphis gra-minumRondani). Segments of infested leaf tissue were fixed, embedded in paraffin, sectioned, and affixed to slides by standard histological techniques. Serial sections were viewed by LM as temporary mounts in xylene. Sections of interest were identified and re-embedded in fingernail polish, affixed to aluminum stubs, freed of polish with ethyl acetate or acetone, and sputter-coated for SEM. SEM of re-embedded leaf sections showed excellent preservation of leaf anatomy. The same aphid tracks and regions of cell damage identified by LM were visible. SEM increased resolution and provided a much clearer sense of the three-dimensional relations involved in the interaction between plant and insect.
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