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Light and Scanning Electron Microscopy of Greenbug Aphid Damage in Wheat Using the Same Section

 

作者: LedfordJoanna H.,   RichardsonPaul E.,  

 

期刊: Biotechnic&Histochemistry  (Taylor Available online 1994)
卷期: Volume 69, issue 6  

页码: 342-347

 

ISSN:1052-0295

 

年代: 1994

 

DOI:10.3109/10520299409106315

 

出版商: Taylor&Francis

 

关键词: greenbug;aphid;wheat;correlative microscopy;scanning electron microscopy;feeding damage

 

数据来源: Taylor

 

摘要:

A method has been developed to enable correlative light microscopy (LM) and scanning electron microscopy (SEM) on the same section of wheat(Triticum aestivumL.) leaves infested by greenbug aphids(Schizaphis gra-minumRondani). Segments of infested leaf tissue were fixed, embedded in paraffin, sectioned, and affixed to slides by standard histological techniques. Serial sections were viewed by LM as temporary mounts in xylene. Sections of interest were identified and re-embedded in fingernail polish, affixed to aluminum stubs, freed of polish with ethyl acetate or acetone, and sputter-coated for SEM. SEM of re-embedded leaf sections showed excellent preservation of leaf anatomy. The same aphid tracks and regions of cell damage identified by LM were visible. SEM increased resolution and provided a much clearer sense of the three-dimensional relations involved in the interaction between plant and insect.

 

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