Enzyme-Amplified Receptor Assay (ERA): A Novel Approach to Drug Detection
作者:
S.F. Hallowed,
G.A. Rechnitz,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 12
页码: 1929-1949
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708078037
出版商: Taylor & Francis Group
关键词: Bioassay;Acetylcholine Receptor;Enzyme Amplification;PCP;Drug Detection
数据来源: Taylor
摘要:
Isolated acetylcholine receptors from Torpedo nobiliana are used as recognition elements for phencyclidine (PCP), a potent hallucinogen with high affinity for the ion channel site associated with the receptor, in a novel enzyme-amplified receptor assay. A fixed amount of PCP labelled enzyme competes with free PCP for a limited number of receptor sites resulting in changes of observed enzyme activity proportional to free drug concentration. The proposed concept combines the use of receptors with an enzyme amplification step to yield an exceptionally simple and convenient assay.
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