Retinyl acetate in soy based infant formula was extracted by matrix solid phase dispersion (MSPD) and chromatographed by isocratic normal phase chromatography on a Si 60 column with a mobile phase of 0.28% (v/v) isopropanol in hexane. Detection was by fluorescence (Ex λ=325, Em λ=470). Fluorescence response was linear (r2=0.999) from 0.10 – 2.5 μg/mL. Recoveries determined on a soy based infant formula zero control reference material (ZRM) containing added analyte at five levels averaged 94.7% (n=25) with CV's from 0.57–3.53%. The method expands the use of MSPD extraction to the analysis of retinylacetate in soy based infant formulas.