Effect of pore diffusion limitation on dextrin hydrolysis by immobilized glucoamylase
作者:
Douglas D. Lee,
Gene K. Lee,
Peter J. Reilly,
Yoon Y. Lee,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1980)
卷期:
Volume 22,
issue 1
页码: 1-17
ISSN:0006-3592
年代: 1980
DOI:10.1002/bit.260220102
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractData reported here and previously indicate that when dextrin is hydrolyzed in the presence of immobilized glucoamylase, use of a larger average molecular weight substrate leads to lower overall rates of hydrolysis, while the maltose concentration during the bulk of the reaction and the maximum glucose concentration are lower than when the soluble form of the enzyme is employed under the same conditions. Computer simulation of the system demonstrated that all three observations were caused by pore diffusion limitation: the first by slow diffusion of substrate, the second by slow diffusion of intermediates, and the third by slow diffusion of glucose. Follow‐up experiments with glucoamylase immobilized to particles of different sizes confirmed this finding, as results with the smallest beads were identical to those with soluble glucoamylas
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