Vaginal Keratinization during the Estrous Cycle in Rats: A Model for Evaluating Retinoid Activity
作者:
Danielle Chateau,
Jean-Marie Geiger,
Brigitte Samama,
Nelly Boehm,
期刊:
Skin Pharmacology and Physiology
(Karger Available online 1996)
卷期:
Volume 9,
issue 1
页码: 9-16
ISSN:1660-5527
年代: 1996
DOI:10.1159/000211385
出版商: S. Karger AG
关键词: Estradiol;Rat;Retinoid;Vaginal smear;Keratinization;Estrous cycle
数据来源: Karger
摘要:
A model is described for evaluating the activity of a retinoid based on its effect on the keratinization of the vaginal epithelium that occurs on estrus (day 4) of a 4-day cycle in female rats. This keratinization process is dependent on the endogenous estradiol (E2) secreted between the evening of diestrus 2 (day 2) and that of proestrus (day 3). Various doses of all-trans-retinoic acid (tRA) were injected at different time points during the estrous cycle and the vaginal keratinization was assessed by microscope examination of unstained native or Papanicolaou-stained smear preparations. Additionally, the preovulatory E2 secretion was measured and ovaries were histologically examined. A single injection of 10 mg/kg tRA either on diestrus 2 (evening) or on proestrus (early morning) was able to induce a complete inhibition of the vaginal keratinization in more than 80% of the cases. This can be considered as a direct effect on the vaginal epithelial differentiation since neither the E2 secretion nor the ovulatory process were affected. The inhibition of vaginal keratinization can be used as a rapid and convenient in vivo model for screening retinoid candidates with antikeratinizing activity.
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