Effects of glucose concentration on cortisol induction of glutamine synthetase in chick embryo retinas
作者:
ReifLiane,
AdlerAlice J.,
期刊:
Current Eye Research
(Taylor Available online 1982)
卷期:
Volume 2,
issue 2
页码: 117-122
ISSN:0271-3683
年代: 1982
DOI:10.3109/02713688208997685
出版商: Taylor&Francis
数据来源: Taylor
摘要:
We report here that cortisol induction of glutamine synthetase in the chick embryo retina is maximal when the glucose concentration in the culture medium is 0.2 g/1, rather than the 1 g/1 (0.1%, or 6 mM) customarily found in many commercially available media; induction is a factor of 2.1 higher at 0.2 g/1. Glutamine synthetase is known to be localized within retinal Müller cells. The pH of the medium at the end of a 24-hr culture period is generally higher (approximately 7.2) in the low-glucose medium than that found in the standard maintenance medium (pH approximately 6.8), probably indicating decreased metabolism at the lower sugar concentration. Manipulation of the culture medium components may provide a method to monitor metabolic stress responses of Müller cells.
点击下载:
PDF (444KB)
返 回