Sensitive Sandwich Enzyme Immunoassay of Human Interleukin-2 ProducedIn Vitroby Peripheral Blood Mononuclear Cells
作者:
Seiichi Hashida,
Eiji Ishikawa,
Hisanori Umehara,
Shunichi Kumagai,
Hiroo Imura,
Yasukazu Ohmoto,
Yoshikatsu Hirai,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 7
页码: 1083-1097
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708064593
出版商: Taylor & Francis Group
关键词: Interleukin-2;Enzyme immunoassay;Peroxidase;Mononuciear cell
数据来源: Taylor
摘要:
A sensitive sandwich enzyme immunoassay for human inter-leukin-2 (hIL-2) using monoclonal antibody IS described. A monoclonai anti-hIL-2 IgG-coated poiystyrene ball was incubated with hIL-2 and subsequently with affinity-purified rabbit anti-hIL-2 Fab'-horseradish peroxidase conjugate. Peroxidase activity bound to the polystyrene ball was assayed by fluorimetry using 3-(4-hydroxyphenyl)propionic acid as a substrate. There was no cross-reaction with interleukins 1α and 1bT, epidermal growth factor, insulin and other protein hormones. The detection limit of hIL-2 was 3 pg/tube or 30 ng/1 using 0.1 ml of cuiture supernatant. When peripheral blood mononuclear cells from healthy subjects aged 22-62 yr were cultured in the absence and presence of phytohemagglutinin P for 48 h, hIL-2 levels in the culture supernatants were <0.03-0.10 μg/1 and 0.18-3.7 μg/1, respectively.
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