Quantification of Tartrate Resistant Acid Phosphatase Activity Using a Computerized Image Analysis System
作者:
KellyJanet Dvonch,
McNultyJohn A.,
SchneiderGary B.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 5
页码: 228-235
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109109977
出版商: Taylor&Francis
关键词: tartrate resistant acid phosphatase;image analysis
数据来源: Taylor
摘要:
Tartrate resistant acid phosphatase (TRAP) has been accepted as a marker for identification of osteoclasts. A method is reported here for quantitating TRAP using an image analysis system. The amount of the enzyme specific to osteoclasts can be used to differentiate osteoclasts from other cells capable of TRAP expression. TRAP expression characteristic of the osteoclast was compared with that of multinucleated giant cells (MNGC)s recruited to the site of subcutaneously implanted mineralized bone matrix. Two weeks post-implantation, the pellets were removed and processed for the demonstration of TRAP along with rat proximal tibiae. A large amount of TRAP was consistently expressed by the in situ osteoclasts. The MNGCs associated with the mineralized bone implants expressed little if any TRAP reaction product. Using this system, the amount of TRAP reaction product or any other enzyme reaction product expressed can be objectively and reproducibly quantitated.
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