We compared the homing ability of three groups of rainbow trout (Salmo gairdneri) stocked in Lake Michigan by different procedures. One group of juvenile rainbow trout was imprinted to a synthetic chemical, morpholine, during the presmolt and smolt stages, and a second group was not imprinted. Both groups were stocked directly into Lake Michigan, 1 km north of the Little Manitowoc River. A third group of trout was retained in a pond on the Little Manitowoc River during the presmolt and smolt stages and then released into Lake Michigan at the same location as the other two groups. During the adult spawning migration, morpholine was metered into the Little Manitowoc River. This river and 16 other locations were monitored for returning fish. The morpholine-imprinted fish returned to the Little Manitowoc River in greater numbers and strayed less than did fish from the other two treatment groups. This result is a consequence of exposure to a unique odor cue at the critical period for imprinting.