Gas Chromatographic Determination of Nordihydroguaiaretic Acid in Larrea Divaricata
作者:
J.L. Valentine,
Larry McKenzie,
Frances Kovarik,
期刊:
Analytical Letters
(Taylor Available online 1984)
卷期:
Volume 17,
issue 14
页码: 1617-1626
ISSN:0003-2719
年代: 1984
DOI:10.1080/00032718408065335
出版商: Taylor & Francis Group
关键词: Nordihydroguaiaretic acid;L. divaricata;Glc;stability
数据来源: Taylor
摘要:
A glc analysis method utilizing a flame ionization detector was developed to determine nordihydroguaiaretic acid (NDGA). Lyophilized samples of an aqueous extract from leaves ofL. divaricatawere reconstituted in water at a concentration of 50 mg/ml. A 100 mcl aliquot of this solution had 50 mcl of the internal standard solution, p,p'-biphenol (0.4 mg/ml in methanol) added followed by extraction with benzene:isopropyl alcohol (98:2), evaporation, and trimethylsilylation with N,O-bis-(trimethylsilyl)-acetamide. Glc analysis was accomplished using a 10% OV-101 on 80/100 Gas Chrom Q column. Accuracy and precision for the glc method was demonstrated over the range of anticipated concentration of NDGA in theL. divaricataleaves. The content of NDGA present in the leaves was found to be 1.6%.
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