ACTIVITY in the human supplementary motor area (SMA), primary motor cortex (M1), and the draining vein of the motor cortex during a visually triggered finger opposition task was measured by functional magnetic resonance imaging with a repetition time of 1 s. Sequential hemodynamic activation in these areas was revealed by cross-correlating a signal sequence in M1 directly with signal sequences from SMA and the draining vein, and applying polynomial fitting with the aid of Akaike's information criterion. We succeeded in detecting a time delay of approximately 0.5 s between the activations of SMA and M1, and a delay of nearly 1.3 s between the activations of M1 and its draining vein. The new combination of fMRI and data analysis techniques has attained a time resolution comparable to those in preceding studies that used shorter fMRI repetition times of 100–200 ms.