Ascorbate oxidation reduction inHelicoverpa zeaas a scavenging system against dietary oxidants
作者:
Gary W. Felton,
Sean S. Duffey,
期刊:
Archives of Insect Biochemistry and Physiology
(WILEY Available online 1992)
卷期:
Volume 19,
issue 1
页码: 27-37
ISSN:0739-4462
年代: 1992
DOI:10.1002/arch.940190104
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: AFR reductase;DHA;DHA reductase;glutathione reductase;quinones;quinone reductase;chlorogenic acid;polyphenol oxidase;antioxidants
数据来源: WILEY
摘要:
AbstractWe provide evidence of an important role for ascorbate free radical (AFR) reductase, dehydroascorbate (DHA) reductase, glutathione, and glutathione reductase as components of an oxidant‐scavenging system in the midgut of larvalHelicoverpa zea. Also, midgutortho‐quinone reductase is a potentially important constituent of the protective system against quinones. The midgut activities of AFR reductase, DHA reductase, glutathione reductase, andortho‐quinone reductase were, respectively, 168, 22.1, 6, and 39.5 nmol/min/mg protein. The relatively high activity of these enzymes in the midgut provides circumstantial evidence for a protective mechanism utilizing ascorbate as an antioxidant and glutathione and/or NADPH as reductants. To our knowledge, the enzymes AFR reductase and DHA reductase have not been reported in insects. The particular relevance of this system to antioxidant protection, and in particular to the detoxication of quinones formed in damaged leaf tissues, is disc
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