Ethylenediaminetetraacetic Acid in Ammonium Hydroxide for Reducing Decalcification Time
作者:
SandersonCathy,
RadleyKaren,
MaytonLois,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1995)
卷期:
Volume 70,
issue 1
页码: 12-18
ISSN:1052-0295
年代: 1995
DOI:10.3109/10520299509108310
出版商: Taylor&Francis
关键词: decalcification;EDTA;NaOH;NH4OH;DMSO;vacuum;weight loss method;X-ray;sonication
数据来源: Taylor
摘要:
Ethylenediaminetetraacetic acid (EDTA) solution is used to decalcify bone specimens for histological examination. Sodium hydroxide (NaOH) has been used to dissolve EDTA and to bring EDTA solutions to neutral pH. This solution, however, requires several weeks to decalcify bone specimens. We investigated a new de-calcification fluid using concentrated ammonium hydroxide (NH4OH) to dissolve EDTA and to adjust the pH to neutral. Decalcification was performed using a magnetic stirrer with and without vacuum, or with a sonic cleaner. Decalcification end point was confirmed using both the weight loss and X-ray methods. After decalcification, specimens were processed through paraffin and sections were stained with hematoxylin and eosin. Decalcification employing NH4OH required an average of six days. Light microscopy indicated good retention of cellular detail.
点击下载:
PDF (1195KB)
返 回