Glutamine is involved in the dependency of brain neuron survival on cell plating density in culture
作者:
Masatomo Watanabe,
Yoshihide Ohe,
Kenji Katakai,
Kenji Kabeya,
Yukihito Fukumura,
Isao Kobayashi,
Kaoru Miyamoto,
Koichi lshikawa,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 10
页码: 2353-2357
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Brain neurons;Cell plating density;Culture;Glutamine;Survival
数据来源: OVID
摘要:
THE mechanism by which neuronal cell viability in culture is dependent on cell plating density is unclear. To address this question, dissociated cells from the neonatal rat cortex were cultured in a chemically defined medium. Medium conditioned with cortical cells plated at high density (2000 cells/mm2) promoted the survival of neurons grown at low cell density (100 cells/mm2) in a dose-dependent manner. Data obtained from molecular sieving suggested that the molecule(s) promoting the survival of neurons was smaller than 1000 Da. Amino acid analysis of the conditioned medium revealed the release of a mass of glutamine from cortical cells in culture. L-Glutamine mimicked the conditioned medium in action promoting the viability of neurons. These findings suggest that the effect of plating density on neuronal cell viability is mediated at least in part by glutamine released from cultured cells.
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